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led light source coolled pe2 with 490 nm/550  (CoolLED Inc)

 
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    Structured Review

    CoolLED Inc led light source coolled pe2 with 490 nm/550
    Led Light Source Coolled Pe2 With 490 Nm/550, supplied by CoolLED Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/led light source coolled pe2 with 490 nm/550/product/CoolLED Inc
    Average 90 stars, based on 1 article reviews
    led light source coolled pe2 with 490 nm/550 - by Bioz Stars, 2026-03
    90/100 stars

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    <t> Qdots </t> molar extinction coefficient ε (cm -1 M -1 determined at 405nm) and dilutions used to achieve a similar level of <t> fluorescence </t> (adapted from [ <xref ref-type= 11 ])." width="250" height="auto" />
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    Qdots molar extinction coefficient ε (cm -1 M -1 determined at 405nm) and dilutions used to achieve a similar level of fluorescence (adapted from [ <xref ref-type= 11 ])." width="100%" height="100%">

    Journal: PLoS ONE

    Article Title: Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections

    doi: 10.1371/journal.pone.0162419

    Figure Lengend Snippet: Qdots molar extinction coefficient ε (cm -1 M -1 determined at 405nm) and dilutions used to achieve a similar level of fluorescence (adapted from [ 11 ]).

    Article Snippet: Having initially settled on a single excitation system with 425nm (PE2-CoolLED) excitation which was found to be optimum for Qdots work, we investigated the possibility of performing multicolour fluorescence combining Qdots with other fluorophores.

    Techniques: Fluorescence

    FFPE section stained with Qdot 655 was imaged successively using mercury bulb, LED405 & Metal Halide source. The images are intensity images at 655nm. Red arrows: specific 655 fluorescence; green arrows: autofluorescence. The image taken with the first exposure to LED405nm illumination. (LED405 (1) ) shows the specific staining with little autofluorescence when the section is illuminated with LED 405nm. Metal halide exposure led to high level autofluorescence and fast photobleaching of the specific fluorescence, confirmed with a second set of images with LED 405nm (LED405 (2) ). Similar results were obtained with all other Qdots.

    Journal: PLoS ONE

    Article Title: Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections

    doi: 10.1371/journal.pone.0162419

    Figure Lengend Snippet: FFPE section stained with Qdot 655 was imaged successively using mercury bulb, LED405 & Metal Halide source. The images are intensity images at 655nm. Red arrows: specific 655 fluorescence; green arrows: autofluorescence. The image taken with the first exposure to LED405nm illumination. (LED405 (1) ) shows the specific staining with little autofluorescence when the section is illuminated with LED 405nm. Metal halide exposure led to high level autofluorescence and fast photobleaching of the specific fluorescence, confirmed with a second set of images with LED 405nm (LED405 (2) ). Similar results were obtained with all other Qdots.

    Article Snippet: Having initially settled on a single excitation system with 425nm (PE2-CoolLED) excitation which was found to be optimum for Qdots work, we investigated the possibility of performing multicolour fluorescence combining Qdots with other fluorophores.

    Techniques: Staining, Fluorescence

    Comparison of fluorescence intensities when illuminating Qdots with mercury light, LED 405nm or LED 425nm.

    Journal: PLoS ONE

    Article Title: Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections

    doi: 10.1371/journal.pone.0162419

    Figure Lengend Snippet: Comparison of fluorescence intensities when illuminating Qdots with mercury light, LED 405nm or LED 425nm.

    Article Snippet: Having initially settled on a single excitation system with 425nm (PE2-CoolLED) excitation which was found to be optimum for Qdots work, we investigated the possibility of performing multicolour fluorescence combining Qdots with other fluorophores.

    Techniques: Comparison, Fluorescence

    Top panel: An example of the autofluorescence intensity images under illumination with 405+460nm is shown for the indicated emission wave lengths which correspond to the peak of emission of the studied Qdots. ROI shows a typical thresholded region of interest selected for quantification of the intensity of autofluorescence. Graphs : The spectra of autofluorescence were found to be fairly similar between 500 and 720nm. However the level of autofluorescence between 530 to 650nm increases with the excitation wave length with PE4000 (A&C). SOLA2, PE2 425 & PE4000 405+460nm lead to similar autofluorescence spectra (D).

    Journal: PLoS ONE

    Article Title: Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections

    doi: 10.1371/journal.pone.0162419

    Figure Lengend Snippet: Top panel: An example of the autofluorescence intensity images under illumination with 405+460nm is shown for the indicated emission wave lengths which correspond to the peak of emission of the studied Qdots. ROI shows a typical thresholded region of interest selected for quantification of the intensity of autofluorescence. Graphs : The spectra of autofluorescence were found to be fairly similar between 500 and 720nm. However the level of autofluorescence between 530 to 650nm increases with the excitation wave length with PE4000 (A&C). SOLA2, PE2 425 & PE4000 405+460nm lead to similar autofluorescence spectra (D).

    Article Snippet: Having initially settled on a single excitation system with 425nm (PE2-CoolLED) excitation which was found to be optimum for Qdots work, we investigated the possibility of performing multicolour fluorescence combining Qdots with other fluorophores.

    Techniques: